Biography
Maximiliano Giraud-Billoud has completed his both PhD in Medicine and Biology (Physiology) from National University of Cuyo, Argentina and is working now in a postdoctoral project from FONDECyT-CONICyT, Chile. He has published 11 papers in reputed journals. The center for regenerative medicine (CAS-UDD) has many publications in the field of stem cell research.
Abstract
Megalin and cubilin are membrane endocytic receptors that interact for endocytosis of a vast variety of filtered plasma proteins in kidney proximal tubule cells. One characteristic of diabetic nephropathy is the increase of albumin filtration and the inhibition of megalin and cubilin mediated albumin endocytosis, leading to increased albuminuria. The objective of our work was to study the influence of mesenchymal stem cells (MSC) therapy on tubular protein transporters in kidney of type 1 and type 2 diabetic animals. We studied by Western Blot the expression of megalin (tissue) and cubilin (tissue and urine) and the functionality of megalin by the quantification of vitamin D-binding protein (BDP, urine) in control animals, type 1 and 2 diabetic animals (C57BL6+STZ and db/db C57BKS mouse, respectively) and treated with MSC (0,5x106cells/animal) type 1 and 2 diabetic animals. The expression of megalin and cubilin decreases in T1 diabetic animals and after 2 weeks of MSC administration the expression increases. In T2 diabetic animals, only megalin decreases significantly its expression and treatment does not increase levels. Proteinuria and BDP concentration are increased in both diabetic models, but only BDP levels decrease in T2 animals after MSC administration. Finally, levels of cubilin in urinary samples are increased in both diabetic models and only decrease significantly in T1 diabetic animals that received the cell therapy. MSC administration in both experimental diabetic models improves the action of tubular protein transporters, however its potential role as a therapy and the physiopathologic mechanisms involved are currently under study.
Biography
Idiberto José Zotarelli Filho has received his PhD from UNESP University in the field of Regenerative Medicine and Tissue Engineering currently, he is working Researcher in the Institute of Cardiovascular Diseases and UNESP University. He has successfully completed his Administrative Responsibilities the researcher. He has received several awards and honors, such as the International Symposium of extracellular matrix - Buzios / Brazil, International Congress of Hematology - Albert Einstein Hospital - Sao Paulo / Brazil and the International Congress of cell therapy stem cells in São Paulo / Brazil. He has published more than 100 peer-reviewed in journals and is an author of 2 books. Also actively participate in the training program in research - RESEARCH COACHING PROGRAM - DURHAM - Duke University - USA under the auspices of the Brazilian Cardiology Society - SBC and I am a member of the Research Ethics Committee - Institute of Cardiovascular Diseases (IMC).
Abstract
Scaffolds of chitosan and collagen can offer a biological niche for the growth of adipose derived stem cells (ADSC). The objective of this work was to characterize the physico-chemical properties of the scaffolds and the ADSC, as well as their interactions to direct influences of the scaffolds on the behavior of ADSC. The methodology included an enzymatic treatment of fat obtained by liposuction by collagenase, ASDC immunophenotyping, cell growth kinetics, biocompatibility studies of the scaffolds analyzed by the activity of alkaline phosphatase (AP), nitric oxide (NO) determination by the Griess-Saltzman reaction, and images of both optical and scanning electron microscopy of the matrices. The extent of the crosslinking of genipin and glutaraldehyde was evaluated by ninhydrin assays, solubility tests and degradation of the matrices. The results showed that the matrices are biocompatible, exhibit physical and chemical properties needed to house cells in vivo and are strong stimulators of signaling proteins (AP) and other molecules (NO) which are important in tissue healing. Therefore, the matrices provide a biological niche for ADSC adhesion, proliferation and cells activities.