Isfahan University of Medical Sciences, Iran
Title: Isolation of stem cells from dental pulp of primary teeth
Biography: Seyed Amir Mousavi
Introduction: Finding an accessible resource is an important goal for stem-cell research. The aim of this study is to isolate the stem cells from dental pulp of deciduous teeth. Method and material: Anterior deciduous teeth from children 6 to 9 years old which were exfoliating normally were used in this experiment. The exfoliated teeth were immediately placed in a normal saline sterile solution containing antibiotics and were kept in 4 C. The dental pulp was isolated in complete sterile condition and then divided into small pieces with a fine scalpel. Then it was put on 4 mg/ml collagenase type 1 for one hour at 37 c for preparation of single cell suspention. The samples have been cultured in MEMenvironment. To prove that these cells are stem cells we used flow cytometry. Results: 1- Rest of pulp of deciduous teeth contains a population of fibroblast like cells. 2-SHED (stem cells from human exfoliated deciduous teeth) show positive response toward CD90 as an excellent mesenchymal marker and show negative response toward CD31 as an endothelial marker. 3- The rate of reproduction in these stem cells was high. 4- SHED has an ability to differentiate into adipocytes andosteocytes. Conclusion: The deciduous teeth can be used as an accessible and great resource of stem cells without having any moral problems in researches involving tissue engineering.