Jian Zhou
Capital Medical University, China
Title: Wnt3a and bmp7 in odontoblastspecification/differentiation and dentin regeneration
Biography
Biography: Jian Zhou
Abstract
New endodontic therapies that attempt to regenerate dental pulp and dentin may provide alternative solutions to traditional procedures. Previously, we have reported that odontogenesis factors, BMP7 and Wnt10a are highly expressed by odontoblasts. Consistently, BMP and canonical Wnt signaling pathways synergistically promote odontogenesis in vitro. To examine Wnt and BMP mediated dental pulp regeneration, we delivered the two factors into endodontically treated porcine incisors. The regenerated dental pulp and dentin were assessed by histological analysis, micro-CT, scanning electronic microscope (SEM), and hardness tests by nanoindentation. The present study aimed to: 1) quantify the regenerated dentin using histological analysis and micro-CT; 2) measure mechanical parameters of regenerated dentin; 3) assess the microstruture of the regenerated dentin by scanning electronic microscope (SEM).We regenerated dentin and dental pulp in endodontically treated teeth by homing and inducing endogenous perioapical cell migration and differentiation into dental pulp cells. Our results demonstrate that the two factors induce dentin regeneration and that the mechanical character and mineral density of regenerated dentin is comparable to the native dentin. This research supports a new approach to endodontic therapy as regenerative pulp and dentin have been shown to function like the native tooth.
