Akshay Anand
Post Graduate Institute of Medical Education and Research India
Title: Does hUCB derived Lin-ve stem cells rescue amyloid-β peptide induced mouse model of memory loss
Biography
Biography: Akshay Anand
Abstract
Objectives: The objective of the study was to evaluate the effect of lineage negative human umbilical cord blood derived stem cells in rescue of memory loss in animal model of AD.
Methods: We used digital stereotaxis, immunohistochemistry, real time PCR and behavioral assays such rota rod and morris water maze in order to evaluate the learning and memory in experimetal and control Swiss Albino mice. Lineage negative stem cells were purified by MACS and labelled by CFDA dye in order to trace the stem cell recruitment, incorporation and differentiation and FACS analysis to characterise these cells before transplantation.
Results: There was dose dependent rescue of memory loss caused by transplantation of lineage negative hUCB cells. There was increase in BDNF and CREB expression subsequent to transplantation of Lineage negative human UCB cells as well as decline in apoptosis analysed by Fas L and Fluro Z. The immunolocalisation of Abeta reduced with transplantation of these cells.
Conclusion: Lineage negative UCB cells possess the necessary neurotrophic effect in extending the survival of hippocampal neurons which may contribute in rescue of memory loss. The immunolocalisation of Abeta reduced with transplantation.